IB PRIME COMPETITION (OCTOBER 2018) WINNING PROJECT: SCOTBIO AND UNIVERSITY OF EDINBURGH

3D-AEX: a novel 3D printed chromatography column for improving the extraction and purification of C-phycocyanin

Spirulina is an established cyanobacterial bioplatform that naturally produces the high value protein pigment C-phycocyanin (CPC). ScotBio currently generates food-grade CPC as a healthy, all-natural blue food colourant (ScotBio BlueTM). CPC is also an important component of the pharmaceutical, nutrition and cosmetic industries with a market value of £88 million that is expected to grow four-fold by 2021. The value of CPC is directly correlated with its purity. We have developed a novel 3D printed Anion Exchange Chromatography column system called 3D-AEX, which can significantly reduce the downsteam processing costs for CPC purification by separating CPC directly from cell lysates. In the current project we will test the robustness of 3D-AEX using industrial biomass supplied by ScotBio, investigate how the quality of biomass influences the characteristics and achievable purity of CPC, and finally scale-up 3D-AEX as a trial for commercial scale operation.

Industry Partner: ScotBio

Academic Partner: University of Edinburgh -Alistair McCormick and Simone Dimartino

IBioIC Funding: £49,981.00

Total Project Cost: £140,637.00